Clinicopathological Characteristics of Gastric Cancer in Iranian Patients Referred to Imam Reza Hospital During 2008 to 2017

Background and Aim: There are a limited number of studies regarding the clinicopathological features of gastric cancer. Here, the clinicopathological features of gastric cancer including tumor type and size, degree of differentiation, tumor operability, and immunohistochemically results of E-cadherin and P53 expression was investigated in Iranian patients. Methods: This retrospective study was performed on patients who were admitted at Imam Reza Hospital in Tehran-Iran from 2008 to 2017 with a diagnosis of gastric cancer. Required information including age, sex, type of cancer, pathology, and immunohistochemistry (IHC) results and treatment were extracted from the hospital archive. Results: Out of 264 enrolled patients (with the mean age of 70.03±14.01 years), 180 cases were men. The frequency of intestinal-type, Diffuse-type, lymphoma and GIST tumor were 54.5%, 27.7%, 12.25% and 5.92% respectively. In addition, 67.58% and 13.83% of the tumors were undifferentiated and poorly differentiated respectively. Surgery was less probable in less differentiated tumors (r=0.582 P=0.001). The surgery rate in GIST, intestinal-type, and Diffuse-type tumors were 100%, 57%, and 14.4%, respectively. IHC results showed that E-cadherin expression was present in 78.6, of which 50.9% were weakly positive (+1). Also, the high expression of P53 was observed in 60.7% of patients. Conclusion: According to the data, we can conclude that poorly differentiated tumors, decreased expression of E-cadherin, and increased expression of P53 is linked to poor prognosis in Iranian patients with gastric cancer. In this regard, further clinical trials and multicenter studies should be done to evaluate the possible factors for improving the prognosis and survival rates of Iranian patients with gastric cancer. *Corresponding Author: Shahrokh Iravani; Email: [email protected] Please cite this article as: Sattartabar B, Nourian M, Samizadeh E, Mehrvar N, Jalaeikhoo H, Mehrvar A, Iravani S. Clinicopathological Characteristics of Gastric Cancer in Iranian Patients Referred to Imam Reza Hospital During 2008 to 2017. Arch Med Lab Sci. 2020;6:1-6 (e18). https://doi.org/10.22037/amls.v6.3330

Assessing Genetic Diversity of Rice Varieties under Drought Stress Conditions

In order to compare different rice genotypes grown under drought stress conditions a field experiment was conducted. In this study thirty different genotypes of native, breeded and upland cultivars were evaluated. Analysis of variance showed significant differences among genotypes in respect of all vegetative and morphological traits. Genotypes were devided into three groups by cluster analysis based on all studied traits with minimum variance method (Ward�s Method). The total average indicates significant differences among groups in respect of all morphological and physiological characteristics. In addition, eight drought stress tolerance indices including: sensitivity to stress index (SSI), drought response index (DRI), relative drought index (RDI), tolerance index (TOL), mean productivity index (MP), stress tolerance index (STI), geometric mean productivity index (GMP) and harmonic mean index (HM) were calculated according to their grain yield under drought stress and normal conditions. In general, results of this experimnet revealed that, among rice cultivars �Domsephid�, �Deylamany�, �Hasansaraei�, �Sadri�, �Anbarboo� and �Domsiah� had the highest sensitivity referring to drought stress and produced the lowest grain yield. Also, genotypes of �IR24� (breeded of IRRI), �Nemat�, �Sephidroud�, �Kadoos� and �Bejar (breeded of Iran) and �Vandana�, upland cultivar (originally from India) had the highest tolerance to drought stress and produced the highest grain yield. In conclusion, it was suggested that, these cultivars are suitable for drought stress conditions and are appropriate for hybridization with the aim of increasing drought tolerance

Assessing Genetic Diversity of Rice Varieties under Drought Stress Conditions

In order to compare different rice genotypes grown under drought stress conditions a field experiment was conducted. In this study thirty different genotypes of native, breeded and upland cultivars were evaluated. Analysis of variance showed significant differences among genotypes in respect of all vegetative and morphological traits. Genotypes were devided into three groups by cluster analysis based on all studied traits with minimum variance method (Wards Method). The total average indicates significant differences among groups in respect of all morphological and physiological characteristics. In addition, eight drought stress tolerance indices including: sensitivity to stress index (SSI), drought response index (DRI), relative drought index (RDI), tolerance index (TOL), mean productivity index (MP), stress tolerance index (STI), geometric mean productivity index (GMP) and harmonic mean index (HM) were calculated according to their grain yield under drought stress and normal conditions. In general, results of this experimnet revealed that, among rice cultivars Domsephid, Deylamany, Hasansaraei, Sadri, Anbarboo and Domsiah had the highest sensitivity referring to drought stress and produced the lowest grain yield. Also, genotypes of IR24 (breeded of IRRI), Nemat, Sephidroud, Kadoos and Bejar (breeded of Iran) and Vandana, upland cultivar (originally from India) had the highest tolerance to drought stress and produced the highest grain yield. In conclusion, it was suggested that, these cultivars are suitable for drought stress conditions and are appropriate for hybridization with the aim of increasing drought tolerance

Genetic Variability, Correlation and Path Analysis in Rice under Optimum and Stress Irrigation Regimes

In order to estimate genetic variability and relationships among some agronomic traits of rice an experiment were conducted with 30 varieties of rice under two irrigation regimes. There were significant differences among the varieties for all traits. Broad-sense heritability varied from 0.05 for brown grain width to 0.99 for plant height and number of spikelet for panicle under optimum irrigation and from 0.1 for brown grain width to 0.99 for plant height. Evaluation of phenotypic and genotypic coefficient of variations (CV) showed that the lowest and highest phenotypic CV under optimum irrigation regime was observed to panicle fertility percentage and paddy yield and genotypic CV was related to brown grain width and plant height, respectively, while under drought stress condition, days to 50% flowering had the lowest phenotypic and genotypic CV and paddy yield and plant height had the highest phenotypic and genotypic CV. Furthermore, the lowest and highest expected genetic advance using selection intensity of 10% (i=1.75) were evaluated for brown grain width and plant height under optimum irrigation regime, respectively. Path analysis for paddy yield indicated that the number of spikelet per panicle and flag leaf length had positive direct effects and days to complete maturity and plant height had negative direct effects on paddy yield under optimum irrigation condition, while flag leaf width and number of filled grains per panicle had positive direct effects and days to 50% flowering had negative direct effect on paddy yield under drought stress condition

Surveying the Reasons for Refusing Coronary Angiography in Patients Referring to Cardiac Ward of Valiasr Hospital in Fasa

Background & Objective: Coronary angiography has become the largest interventional diagnostic test in recent years. As it has had large effects on anxiety and satisfaction of patients in treatment and care; the objective of the present study is to survey the reasons for which the patients, referring to cardiac ward of Valiasr hospital in Fasa, refuse to undergo coronary angiography.  Materials & Methods: In this cross-sectional study, 60 individuals candidate for coronary angiography were selected. The samples were selected via census. The tool for collecting the data was a researcher-made questionnaire. The data were analyzed by SPSS-15 software, descriptive statistics, Mann-Whitney test, and Kruskal- Wallis test.  Results: The mean age of the samples was 58/9±12/8 years. Financial problems, fear and anxiety, lack of awareness, and lack of confidence in the physician’s diagnosis were the most important factors. The effect of fear has been reported more in women compared to men for refusing coronary angiography (p=.037).  Conclusion: According to the above results, financial problems, fear and anxiety, lack of awareness, and lack of confidence in the physician’s diagnosis are the factors effective in accepting coronary angiography. Therefore, more contrivances are needed to eliminate these effective factors; in order to prepare cardiac patients, who are candidate for coronary angiography, a tranquility to accept and to perform angiography

Diagnostic Accuracy of Immunologic Biomarkers for Accurate Diagnosis of Bloodstream Infection in Patients with Malignancy: Procalcitonin in Comparison with C-Reactive Protein

Purpose. To calculate the diagnostic value of C-reactive protein (CRP) and serum procalcitonin (PCT) levels for the pathologic presence of microbes in the bloodstream of patients with malignancy, in comparison with blood culture. Methodology. Blood culture (by reference method) and assay results of PCT and CRP of febrile patients, with clinical suspicion to blood infections, were collected. Statistical aspects of PCT  and CRP tests were evaluated. Results. Data from 255 cases were gathered. The area under the curve for differentiating bacteremia from nonbacteremia for PCT (0.741) was superior to that of CRP (0.612). Amongst the different cutoffs of PCT and CRP, the cutoff of ≥1.17 ng/ml and >47 mg/l had the sensitivity of 75 and 58.3%, the best NPV of 91.5% and 81.3%, and the best specificity of 79.9% and 72.8%, respectively. Discussion. Despite statistically nonsignificant results, PCT seems to be a superior indicator to CRP for rejecting the presence of microorganism in bloodstream. For PCT, the cutoff value of 1.17 ng/ml (bacteremia from nonbacteremia) had the highest NPV value of 91.5% in malignant patients, suspicion of sepsis

Assessment of Rice Genotypes Response to Drought Stress at the early Reproductive Stage Using Stress Tolerance Indices

Drought stress is one of the major abiotic stresses that limits rice productivity in the world. In this study 83 diverse rice genotypes were evaluated under reproductive-stage drought stress and non-stress conditions in a completely randomized design with three replications. Eleven well known stress tolerance and susceptibility indices were calculated based on the grain yield under stress and non-stress conditions. Also rank mean (RM) indices were calculated based on the ranks of genotypes for the former calculated indices. The highest seed yield under non-stress condition belonged to the genotypes Sangjo, Zenith, IR70445-146-3-3, Norin 22 and Ghasroldashti. Under stress condition, however, IR83750-131-1, IR74718-24-2-3, Sepidrood, TETEP and IR71739-24-3-5 genotypes showed the highest seed yield. STI, GMP, MRP and REI indices with positive and significant correlations with grain yield in stress and non-stress conditions were found to be the most suitable indices for selection of drought tolerant rice genotypes. According to these indices, IR83750-131-1, Sangjo, Sepidrood, Zenith and IR58 genotypes showed the highest ranks and had a higher tolerance to drought stress. Based on RM index, IR83750-131-1, IR74718-24-2-3, Sepidrod, TETEP and IR74720-13-1-2 obtained the highest ranks. Cluster analysis grouped the most drought tolerant genotypes in the second cluster

Analysis and comparison of fragrant gene sequence in some rice cultivars

It is known that the fragrant trait in rice (Oryza sativa L.) is largely controlled by fgr gene on chromosome 8 and it has been specified that the existence of an 8 bp deletion and three single nucleotide polymorphism (SNP) in exon 7 is effective on this trait. In this study, sequence alignment analysis of fgr exon7 on chromosome 8 for 11 different fragrant and non-fragrant cultivars revealed that 5 aromatic rice cultivars carried 3 SNPs and 8 bp deletion in exon7 which terminates prematurely at a TAA stop codon. However, 5 of the non-aromatics showed a sequence identical to the published Nipponbare, being non-fragrant Japonica variety sequence. An exception among them was Bejar, which had 8 bp deletion and 3SNPs but it was non-aromatic. Sequencing can determine nucleotide alignment of a gene and give beneficial information about gene function. In silico prediction showed proteins sequences alignment of fgr gene for Khazar and Domsiah genotypes were different. Betaine aldehyde dehydrogenase complete enzyme belongs to Khazar non-fragrant genotype that has complete length and 503 amino acids while non-functional BADH2 enzyme for Domsiah fragrant genotype has 251 amino acids that result in accumulate 2-acetyl-1-pyrroline (2AP) and produces aroma in fragrant genotypes

Evaluation of Antibacterial Activity and Total Phenol Compounds of Punica granatum Hydro-Alcoholic Extract

Background & Objectives: Punica granatum is a non-productive form of a plant and is used for the treatment of diseases in traditional medicine. In this study, we evaluate the antibacterial activity and the total phenol compounds of Punica granatum. Materials & Methods: Disk and well diffusion methods and MIC were used to evaluate the antibacterial activity of hydro-alcoholic extract on S. aureus and E. coli compared to standard commercial antibiotic disks. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods (Folin-ciocalteu indicator). Results: 35 and 29 mm inhibition zones in S. aureus and 22 and 17 mm inhibition zones in E. coli were shown by disk and well diffusion method, respectively. Also, 7.8 mg/ml concentration of extract showed the MIC points for two bacteria. Phenol compound of extract was 233.15±5.1 mg/g of extraction. Conclusion: Antibacterial effect of Punica granatum compared to antibiotics indicates the strong activity against examined bacteria. Extensive antibacterial study of Punica granatum is suggested

Surveying the Effect of the Phenol Compounds on Antibacterial Activity of Herbal Extracts: In vitro Assessment of Herbal Extracts in Fasa-Fars Province

Background & Objectives: Due to increase in  bacterial drug resistance, discovering new antibacterial compounds is really important. The objective of this study is to evaluate the phenol compounds effect on antibacterial activity of herbal extracts of Fasa-Fars province in vitro. Materials & Methods: The antibacterial activity of 26 plants was studied by disk diffusion, well, and MIC methods in compare with 13 standard antibiotics against S. aureus and E. coli as control bacteria. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods using Folin-ciocalteu indicator. Results: Inhibition zone of bacterial growth  against S. aureus in well and disk methods were 32 and 22 mm in using Zataria multiflora, respectively .And there were 23 and 16 mm against E. coli in Zataria multiflora, respectively. Less effects and inhibition zones, less than 15mm on both strains, were seen in using  Saturina hortensis, Cinamomum zeylanicum, ­Artemisia absinthium, ­Urtica dioica, Carum carvi L. cyminum Cuminum, Achillea fragrantissimia, Marticaria chamomilla, Zingiber officinale, Origanum majorana, and Plantago psyllium. Most effective MIC results, 7.8 µg/ml, were related to the extracts of Zataria multiflora, Carum copticum L. Rosmarinus officinalis L., and Laurus nobilis L. Phenol compound amounts were approximately between 66.51±1.9 and 233.15±5.1 mg/gr extract in Zataria multiflora and Plantago psyllium, respectively. Conclusion: Results of antibacterial activity of extracts and relation with phenol compound amounts indicate the antibacterial effect of phenol compounds in herbal extracts